GSM1286175: d4 M2 kd; Mus musculus; RNA-Seq - SRA

SRX396275: GSM1286175: d4 M2 kd; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 27.3M spots, 1.4G bases, 962.8Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Small RNAs in undifferentiated and differentiating mouse embryonic stem cells

PRJNA171949 • SRP014691 • All experiments • All runs

show Abstracthide Abstract

We report the identification of small RNAs in undifferentiated (d0) and differentiating (d4) mouse embryonic stem (ES) cells using high-throughput sequencing. The goal of this study was to identify small RNAs involved in X-chromosome inactivation (XCI). We have identified a subset of small RNAs that are generated from transposon sequences and map to the X-chromosome, suggesting their involvement in transposon control on the inactivating X-chromosome. Overall design: Examination of 5 small RNA and 4 RNA-seq libraries in 3 ES cell states.

Sample: d4 M2 kd

SAMN02469167 • SRS517302 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: The NEBNext Small RNA Sample Prep Set 1 (Illumina Compatible) library construction protocol was followed (NEB #E6120S/L), along with the SMART kit and Nextera kit for Illumina sequencing for RNA-seq libraries.

Experiment attributes:

GEO Accession: GSM1286175

Links:

External link: GEO Sample GSM1286175

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 27.3M spots, 1.4G bases, 962.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR1055372	27,343,995	1.4G	962.8Mb	2018-03-22

ID:: 570354

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